Generator
Part:BBa_K4144006:Design
Designed by: Boshuo Zhou Group: iGEM22_WHU-China (2022-10-02)
This part is designed to constitutively express XylR, a repressor suppressing promoter Pxyl
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is constructed to ensure the xylose-induced promoter works.
Source
The XylR fragment is gained through PCR from Bacillus subtilis genome, and the P43 is gained from chemical synthesis.