Generator

Part:BBa_K4144006:Design

Designed by: Boshuo Zhou   Group: iGEM22_WHU-China   (2022-10-02)


This part is designed to constitutively express XylR, a repressor suppressing promoter Pxyl


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is constructed to ensure the xylose-induced promoter works.


Source

The XylR fragment is gained through PCR from Bacillus subtilis genome, and the P43 is gained from chemical synthesis.

References